Illumina nextseq 500 user manual

At illumina, our goal is to apply innovative technologies to the analysis of. Illumina exome illumina dna prep with enrichment replicates of na12878. Whole genome library prep for illumina multiplexed sequencing. Trusight oncology 500 highthroughput illumina, inc. Chapter1overview introduction 1 additionalresources 1 instrumentcomponents 2 sequencingconsumablesoverview 5 introduction theillumina nextseq. Access costefficient sequencing, even for low numbers of samples. Page 2 nextseq 550dx research mode instrument reference guide this document and its contents are proprietary to illumina, inc. Illumina dna prep uses a fast, user friendly workflow.

Miniseq reagent kits bring the power of illumina ngs to labs of all sizes. These sequencing reagent kits offer increased stability and robustness over the v2 kit version. Why the next great technology breakthrough shouldnt need a user manual. Additional resources illumina website for access to documentation, software downloads, online training, and frequently asked questions. If you own a ge appliance, its important to have an owners manual to ensure proper maintenance and to answer any questions you may have. Ampliseq for illumina ondemand ampliseq for illumina custom dna panel nextera rapid capture custom enrichment kit nextera flex for enrichment. Features highthroughput sequencing the nextseq 500 enables sequencing of exomes, whole genomes, and transcriptomes and supports truseq and nextera libraries.

We explain how and compare the best share dealing platforms. Ampliseq for illumina on demand panels provide a choice of 5000 pretested genes with known content relevant for inherited disease research, including hereditary cancer, primary immunodeficiency, hearing loss, and muscular dystrophy. The illumina nextseq 550dx highoutput reagent kit v2 300 cycle is a set of reagents and consumables intended for sequencing of sample libraries when used with validated assays. Our existing truseq nano dna library prep kits cat. System overview and instructions for operating and maintaining the nextseq 500. View online or download illumina nextseq 500 system manual. Illumina performance qualification pq services carry out numerous experiments for each system to ensure critical components are tested and validated, have cutoff metrics, and to confirm these metrics show the system is operating in accordance with. Early rate through december 4 technology is part of a modern fascination wi. Tech startups should aspire to foster an intuitive relationship with technology and not get distracted by wiz bang promises.

Page 2 nextseq 500 system guide this document and its contents are proprietary to illumina, inc. Trusight oncology 500 is a nextgeneration sequencing ngs assay that enables inhouse comprehensive genomic profiling of tumor samples. Nextseq 550 system guide 15069765 instructions for using the nextseq 550 system for sequencing and array scanning. As a result, the guide may make assumptions about th. The trusight oncology 500 highthroughput assay is built on the foundation of the trusight oncology 500 assay, with flexibility for higher sample throughput and batching options. Ampliseq for illumina comprehensive panel v3 161 cancer. Accelerate your cytogenomics research for constitutional disorders and cancer. Sureselectqxt whole genome library prep for illumina. At illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that. If you need a replacement owners manual for a toyota car or light truck, its extremely easy to get a. The illumina covidseq test is a nextgeneration sequencing ngs in vitro diagnostic test on the illumina novaseq 6000 sequencing system intended for the qualitative detection of sarscov2 rna from nasopharyngeal np swabs, oropharyngeal op swabs, anterior nasal swabs, midturbinate nasal swabs, nasopharyngeal washaspirates, nasal aspirates, and bronchoalveolar lavage bal specimens from.

Save time, money, and sample by consolidating multiple tests. Illumina covidseq test sarscov2 ngs test for the covid. Your toyota user manual provides important information for safe operation and routine maintenance for your car, truck or other equipment. Supporting the nextseq, miseq, and hiseq sequencing systems. Fc1214001, fc1214002, and fc1214003 will transition to truseq dna nano cat. D to understand, software user manuals are sometimes written from the point of view of a developer rather than a user. Leveraging the power of the novaseq 6000 system for high depth of sequencing, unique molecular identifiers umis, and the ultrarapid illumina dragen server v3, provides high sensitivity and specificity to enable comprehensive genomic profiling from cfdna. Find the system guide, site prep guide, technical notes, and other supporting documentation. An ngsbased assay that assesses multiple variant types in 523 cancerrelated genes from cellfree dna cfdna.

Breathe easier with our openairways guide to better workouts, less coughing and wheezing, and just maybe a longer life. Weve all been thereyou moved to a new home or apartment, and its time to set up electronics and components. The kit is intended for use with the nextseq 550dx instrument and analytical software. Ampliseq for illumina comprehensive cancer panel assess.

Download illumina nextseq 500 system manual manualslib. General electric ge appliances offers consumer home appliances. The illumina covidseq test is a nextgeneration sequencing ngs in vitro diagnostic test on the illumina novaseq 6000 sequencing system, nextseq 500 sequencing system, nextseq 550 sequencing system, or nextseq 550dx instrument intended for the qualitative detection of sarscov2 rna from nasopharyngeal np swabs, oropharyngeal op swabs, anterior nasal swabs, midturbinate nasal swabs. The illumina covidseq test ruo version can be scaled up or down to accommodate different numbers of samples. Continued use of proven v2 reagent and buffer cartridges.

Study the coding and noncoding transcriptome with unparalleled flexibility. Illumina rna prep with enrichment can be used with the illumina exome panel, which features a highly optimized probe set that delivers comprehensive coverage of coding rna sequences. Page 2 nextseq 550dx instrument reference guide this document and its contents are proprietary to illumina, inc. Nextseq 2000 reagent kits contain a reagent cartridge, flow cell, and resuspension buffer with tween 20. Illumina rna prep with enrichment rapid solution for. For questions about willmakers documents and interviews, see also willmaker faqs. Custom content from 1 24 amplicons to 500 15,000 amplicons genes. Nextseq 500 system nextseq 550 system nextseq 550dx system nextseq system nextseq 2000 system miniseq. The flexible nextseq 550 system offers a seamless transition between highthroughput sequencing and array scanning. The comprehensive panel v3 is part of an integrated workflow that includes ampliseq for illumina polymerase chain reaction pcrbased library preparation, illumina sequencing by synthesis sbs nextgeneration sequencing ngs technology, and automated analysis. Library prep kit for illumina hiseq, miseq, and nextseq platforms 16 samples 96 samples agilent pn g9684a pn g9684b ampure xp kit 5 ml. Nextseq 500, nextseq 550, nextseq 550dx in research mode. View options nextseq 500 and nextseq 550 sequencing systems safety and compliance guide 15046564. Get smooth, soft, youngerlooking skin with these skin tips from top dermatologists.

This document and its contents are proprietary to illumina, inc. Illumina operational qualification oq services carry out numerous experiments for each system to ensure critical components are tested and validated, have cutoff metrics, and to confirm these metrics show the system is operating in accordance with specifications. Nextseq 500 system nextseq 550 system nextseq 550dx system nextseq system nextseq 2000 system. Illumina performance qualification pq services carry out numerous experiments for each system to ensure critical components are tested and validated, have cutoff metrics, and to confirm these metrics show the system is operating in accordance with specifications. Illumina advantage largescale sequencing products feature lotspecific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency. Introduction the illumina nextseq 550dx instrument is a single solution that provides a seamless transition between highthroughput sequencing and array scanning. Illumina to sequence genomes for ukwide covid19 study. Laboratory equipment illumina nextseq 550dx instrument reference manual 73 pages. Benefit from the sequencing power of the system, then confirm. Illumina dna prep flexibility for many wholegenome. Multiple flow cell configurations offer adjustable output based on project needs. The comprehensive cancer panel is part of an integrated workflow that includes ampliseq for illumina polymerase chain reaction pcrbased library preparation, illumina sequencing by synthesis sbs nextgeneration sequencing ngs technology, and automated analysis. Comprehensive qualification services from illumina. The illumina covidseq test is a nextgeneration sequencing ngs in vitro diagnostic test on the illumina novaseq 6000 sequencing system, nextseq 500 sequencing system, nextseq 550 sequencing system, or nextseq 550dx instrument intended for the qualitative detection of sarscov2 rna from nasopharyngeal np swabs, oropharyngeal op swabs, anterior nasal swabs, midturbinate nasal.

Ampliseq for illumina sample id panel is a human snp genotyping panel used to generate unique ids for each research sample. Ampliseq library equalizer for illumina is an easytouse solution for normalizing libraries while using ampliseq for illumina library prep methods. Illumina innovative sequencing and array technologies are fueling. The illumina nextseq 500 system combines the power of highthroughput sequencing with the simplicity of a desktop sequencing instrument. Use this online manual answers basic questions about using quicken willmaker plus. Illumina operational qualification oq services carry out numerous experiments for each system to ensure critical components are tested and validated, have cutoff metrics, and to confirm these metrics show. Illumina nextseq 500 system manual pdf download manualslib. Illumina nextseq 550dx instrument reference manual pdf.

Multiple options for both sequencing output and read. Except, when you bought them, you didnt think youd need the user manuals after initially setting them up. Ampliseq for illumina ondemand choose from over 5000. See how researchers are using the nextseq 500 and nextseq 550 systems and access related product literature, videos, and webinars. Illumina stranded total rna prep with ribozero plus. Nextseq system safety and compliance guides documentation.

The illumina exome panel includes 425,000 probes, each constructed against the ncbi37hg19 reference genome, covering 98% of the refseq exome. The illumina covidseq test is a nextgeneration sequencing ngs in vitro diagnostic test on the illumina novaseq 6000 sequencing system intended for the qualitative detection of sarscov2 rna from nasopharyngeal np swabs, oropharyngeal op swabs, anterior nasal swabs, midturbinate nasal swabs, nasopharyngeal washaspirates, nasal aspirates, and bronchoalveolar lavage bal specimens. Nextseq 550 ruo software suite installer release notes documentation. Assay targeting multiple variant types, including tumor mutational burden tmb and microsatellite instability msi. Often filled with jargon, acronyms, and directions that require a ph. Better workouts, less coughing and wheezing, even a longer life. Safety considerations, compliance markings, and regulatory statements for the nextseq 500 and nextseq 550 systems. Nextseq 550dx reagents ivd sequencing illumina, inc. Accelerate your cytogenomics research for constitutional disorders and cancer with the nextseq 550 system. Custom protocol selector generates customized, endtoend instructions. It supports identification of all relevant dna and rna variants implicated in various solid tumor types.

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